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Table 1 Kinetic parameters of PobA mutants towards 4-HBA and 3,4-DHBA

From: A high-throughput visual screening method for p-hydroxybenzoate hydroxylase to increase phenolic compounds biosynthesis

PobA mutants 4-HBA 3,4-DHBA
Vmax
(μM s−1)
Km
(μM)
kcat
(s−1)
kcat/Km
(μM−1 s−1)
Vmax
(μM s−1)
Km
(μM)
kcat
(s−1)
kcat/Km
(μM−1 s−1)
Chen et.al
 Wild type 0.350 ± 0.04 34.7 ± 9.5 14.1 ± 1.5 0.41
 Y385F 0.200 ± 0.001 19.6 ± 0.3 0.200 ± 0.010 0.01 0.390 ± 0.060 228 ± 54 0.390 ± 0.06 0.002
 Y385F/T294A 0.450 ± 0.010 48.4 ± 3.0 0.900 ± 0.030 0.02 0.840 ± 0.090 157 ± 31 1.69 ± 0.18 0.012
This study
 Y385F/T294A 0.800 ± 0.02 89.9 ± 11.2 1.60 ± 0.05 0.018 0.790 ± 0.12 128 ± 52 1.59 ± 0.32 0.012
 Y385F/T294A/V349A 0.680 ± 0.02 14.5 ± 2.5 1.36 ± 0.04 0.094 0.890 ± 0.08 30.3 ± 10.4 1.78 ± 0.16 0.059