Table 1 Kinetic parameters of PobA mutants towards 4-HBA and 3,4-DHBA
PobA mutants | 4-HBA | 3,4-DHBA | ||||||
|---|---|---|---|---|---|---|---|---|
Vmax (μM s−1) | Km (μM) | kcat (s−1) | kcat/Km (μM−1 s−1) | Vmax (μM s−1) | Km (μM) | kcat (s−1) | kcat/Km (μM−1 s−1) | |
Chen et.al | ||||||||
 Wild type | 0.350 ± 0.04 | 34.7 ± 9.5 | 14.1 ± 1.5 | 0.41 | – | – | – | – |
 Y385F | 0.200 ± 0.001 | 19.6 ± 0.3 | 0.200 ± 0.010 | 0.01 | 0.390 ± 0.060 | 228 ± 54 | 0.390 ± 0.06 | 0.002 |
 Y385F/T294A | 0.450 ± 0.010 | 48.4 ± 3.0 | 0.900 ± 0.030 | 0.02 | 0.840 ± 0.090 | 157 ± 31 | 1.69 ± 0.18 | 0.012 |
This study | ||||||||
 Y385F/T294A | 0.800 ± 0.02 | 89.9 ± 11.2 | 1.60 ± 0.05 | 0.018 | 0.790 ± 0.12 | 128 ± 52 | 1.59 ± 0.32 | 0.012 |
 Y385F/T294A/V349A | 0.680 ± 0.02 | 14.5 ± 2.5 | 1.36 ± 0.04 | 0.094 | 0.890 ± 0.08 | 30.3 ± 10.4 | 1.78 ± 0.16 | 0.059 |