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Table 2 Plasmids and strains used in this study

From: A high-throughput visual screening method for p-hydroxybenzoate hydroxylase to increase phenolic compounds biosynthesis

Plasmids and strains Description Source
Plasmids
 pZE12-luc PLlacO1; colE1; ampr Storage
 pCS-PDC PLlacO1-PDC; P15A; kanr Storage
 pETDuet-1 PT7; pBR322; ampr Storage
 pKD46 ParaB-gam-beta-exo; pCS101; ampr Storage
 pCP20 PλpR-flp; pCS101; ampr; cmr Storage
 pZE-PobA** PLlacO1-Y385F/T294A PobA; colE1; ampr This study
 pZE-PobA*** PLlacO1-Y385F/T294A/V349A PobA; colE1; ampr This study
 pETDuet-PobA** PT7-Y385F/T294A PobA; pBR322; ampr This study
 pETDuet-PobA*** PT7-Y385F/T294A/V349A PobA; pBR322; ampr This study
 pZE-AroZ PLlacO1-AroZ; colE1; ampr This study
 pZE-AroZ-PobA** PLlacO1-AroZ; PLlacO1-Y385F/T294A PobA; colE1; ampr This study
 pZE-AroZ-PobA*** PLlacO1-AroZ; PLlacO1-Y385F/T294A/V349A PobA; colE1; ampr This study
E. coli strains
 XL10-Gold TetrD(mcrA)183 D(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1recA1 gyrA96 relA1 lac Hte [Fʹ proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr] Storage
 BW25113 (Fʹ) rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 Fʹ[traD36 proAB lacIqZΔM15 Tn10(Tetr)] Storage
 BL21(DE3) FompT hsdS (rBmB) gal dcm (DE3) Storage
 CTT1 BW25113 (Fʹ) with pZE-PobA** This study
 CTT2 BW25113 (Fʹ) with pZE-PobA*** This study
 CTT3 BW25113 (Fʹ) with pZE-PobA** and pCS-PDC This study
 CTT4 BW25113 (Fʹ) with pZE-PobA*** and pCS-PDC This study
 CTT5 BW25113 (Fʹ)ΔaroE This study
 CTT6 BW25113 (Fʹ)ΔaroEΔydiB This study
 CTT7 BW25113 (Fʹ) with pZE-AroZ This study
 CTT8 CTT5 with pZE-AroZ This study
 CTT9 CTT6 with pZE-AroZ This study
 CTT10 BW25113 (Fʹ) with pZE-AroZ-PobA** This study
 CTT11 CTT5 with pZE-AroZ-PobA** This study
 CTT12 CTT6 with pZE-AroZ-PobA** This study
 CTT13 BW25113 (Fʹ) with pZE-AroZ-PobA*** This study
 CTT14 CTT5 with pZE-AroZ-PobA*** This study
 CTT15 CTT6 with pZE-AroZ-PobA*** This study
 CTT16 BW25113 (Fʹ) with pZE-AroZ-PobA** and pCS-PDC This study
 CTT17 CTT5 with pZE-AroZ-PobA** and pCS-PDC This study
 CTT18 CTT6 with pZE-AroZ-PobA** and pCS-PDC This study
 CTT19 BW25113 (Fʹ) with pZE-AroZ-PobA*** and pCS-PDC This study
 CTT20 CTT5 with pZE-AroZ-PobA*** and pCS-PDC This study
 CTT21 CTT6 with pZE-AroZ-PobA*** and pCS-PDC This study