Table 2 Plasmids and strains used in this study
Plasmids and strains | Description | Source |
|---|---|---|
Plasmids | ||
 pZE12-luc | PLlacO1; colE1; ampr | Storage |
 pCS-PDC | PLlacO1-PDC; P15A; kanr | Storage |
 pETDuet-1 | PT7; pBR322; ampr | Storage |
 pKD46 | ParaB-gam-beta-exo; pCS101; ampr | Storage |
 pCP20 | PλpR-flp; pCS101; ampr; cmr | Storage |
 pZE-PobA** | PLlacO1-Y385F/T294A PobA; colE1; ampr | This study |
 pZE-PobA*** | PLlacO1-Y385F/T294A/V349A PobA; colE1; ampr | This study |
 pETDuet-PobA** | PT7-Y385F/T294A PobA; pBR322; ampr | This study |
 pETDuet-PobA*** | PT7-Y385F/T294A/V349A PobA; pBR322; ampr | This study |
 pZE-AroZ | PLlacO1-AroZ; colE1; ampr | This study |
 pZE-AroZ-PobA** | PLlacO1-AroZ; PLlacO1-Y385F/T294A PobA; colE1; ampr | This study |
 pZE-AroZ-PobA*** | PLlacO1-AroZ; PLlacO1-Y385F/T294A/V349A PobA; colE1; ampr | This study |
E. coli strains | ||
 XL10-Gold | TetrD(mcrA)183 D(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1recA1 gyrA96 relA1 lac Hte [Fʹ proAB lacIqZΔM15 Tn10 (Tetr) Amy Camr] | Storage |
 BW25113 (Fʹ) | rrnBT14 ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 Fʹ[traD36 proAB lacIqZΔM15 Tn10(Tetr)] | Storage |
 BL21(DE3) | F−ompT hsdS (rB−mB−) gal dcm (DE3) | Storage |
 CTT1 | BW25113 (Fʹ) with pZE-PobA** | This study |
 CTT2 | BW25113 (Fʹ) with pZE-PobA*** | This study |
 CTT3 | BW25113 (Fʹ) with pZE-PobA** and pCS-PDC | This study |
 CTT4 | BW25113 (Fʹ) with pZE-PobA*** and pCS-PDC | This study |
 CTT5 | BW25113 (Fʹ)ΔaroE | This study |
 CTT6 | BW25113 (Fʹ)ΔaroEΔydiB | This study |
 CTT7 | BW25113 (Fʹ) with pZE-AroZ | This study |
 CTT8 | CTT5 with pZE-AroZ | This study |
 CTT9 | CTT6 with pZE-AroZ | This study |
 CTT10 | BW25113 (Fʹ) with pZE-AroZ-PobA** | This study |
 CTT11 | CTT5 with pZE-AroZ-PobA** | This study |
 CTT12 | CTT6 with pZE-AroZ-PobA** | This study |
 CTT13 | BW25113 (Fʹ) with pZE-AroZ-PobA*** | This study |
 CTT14 | CTT5 with pZE-AroZ-PobA*** | This study |
 CTT15 | CTT6 with pZE-AroZ-PobA*** | This study |
 CTT16 | BW25113 (Fʹ) with pZE-AroZ-PobA** and pCS-PDC | This study |
 CTT17 | CTT5 with pZE-AroZ-PobA** and pCS-PDC | This study |
 CTT18 | CTT6 with pZE-AroZ-PobA** and pCS-PDC | This study |
 CTT19 | BW25113 (Fʹ) with pZE-AroZ-PobA*** and pCS-PDC | This study |
 CTT20 | CTT5 with pZE-AroZ-PobA*** and pCS-PDC | This study |
 CTT21 | CTT6 with pZE-AroZ-PobA*** and pCS-PDC | This study |