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Table 1 Comparison of 1,3-PDO production by the mono-culture and the CB:KP co-culture at different inoculation ratios under anaerobic serum vial level (20 g/L initial glycerol)

From: Improvement of 1,3-propanediol production from crude glycerol by co-cultivation of anaerobic and facultative microbes under non-strictly anaerobic conditions

Microorganisms

RG (g/L)

1,3-PDO (g/L)

Butyrate (g/L)

Acetate (g/L)

Lactate (g/L)

Succinate (g/L)

Ethanol (g/L)

C. butyricum DUT1a

0.35 ± 0.12

10.79 ± 0.18

2.49 ± 0.21

–

0.23 ± 0.08

–

–

C. butyricum DUT1

19.88 ± 0.34

–

–

–

–

–

–

K. pneumoniae DUT3

1.25 ± 0.13

6.72 ± 0.34

–

1.03 ± 0.12

2.16 ± 0.34

0.71 ± 0.13

1.09 ± 0.10

Syn-CB:KP (4:1)

0.78 ± 0.19

6.41 ± 0.19

–

0.56 ± 0.07

1.72 ± 0.15

0.32 ± 0.09

0.89 ± 0.21

Syn-CB:KP (3:2)

1.56 ± 0.23

6.84 ± 0.27

–

0.43 ± 0.11

2.31 ± 0.23

0.34 ± 0.12

0.91 ± 0.07

Syn-CB:KP (2:3)

1.13 ± 0.17

6.62 ± 0.08

–

0.48 ± 0.09

2.41 ± 0.09

0.36 ± 0.10

1.00 ± 0.27

Syn-CB:KP (1:4)

1.27 ± 0.15

6.67 ± 0.34

–

0.56 ± 0.19

2.46 ± 0.21

0.35 ± 0.13

0.82 ± 0.31

  1. aAnaerobic conditions: 0.2 vvm N2 supply for 3 min
  2. RG, residual glycerol; –, not detectable