Skip to main content

Table 3 Primers designed for C. butyricum DUT1, K. pneumoniae DUT2 and E. coli DUT3

From: Improvement of 1,3-propanediol production from crude glycerol by co-cultivation of anaerobic and facultative microbes under non-strictly anaerobic conditions

Primer

Sequences(5′–3′)

Expected size (bp)

Targeted strain

HgF

AAGAAGCTTTAGAAGATCCTAA

261

C. butyricum DUT1

HgR

GGACAACATGAGGTAAACATTG

261

C. butyricum DUT1

phoEF

TGCCCAGACCGATAACTTTA

210

K. pneumoniae DUT2

phoER

CTGTTTCTTCGCTTCACGG

210

K. pneumoniae DUT2

ybbWF

TGATTGGCAAAATCTGGCCG

210

E. coli DUT3

ybbWR

GAAATCGCCCAAATCGCCAT

210

E. coli DUT3

Back to article page