Table 1 Strains and plasmids
Strains or plasmids | Relevant genotype and description | References |
|---|---|---|
K. pneumoniae strains | ||
 CGMCC 1.6366 | TUAC01 Wild type | [12] |
 ΔbudA–ΔldhA–ipdC | ΔbudA, ΔldhA, pDK6-ipdC | This work |
 ΔbudA–ΔldhA–kivD | ΔbudA, ΔldhA, pDK6-kivD | This work |
 IpdC | ΔbudA, ΔldhA, ΔipdC, pDK6-ipdC | [18] |
 KivD | ΔbudA, ΔldhA, ΔipdC, pDK6-kivD | This work |
 T290L | ΔbudA, ΔldhA, ΔipdC, pDK6-ipdC-T290L | This work |
 L546W | ΔbudA, ΔldhA, ΔipdC, pDK6-ipdC-L546W | This work |
E. coli strains | ||
 E. coli DH5α | Host of plasmid | Lab stock |
 BL21(DE3) | Host of plasmid | Lab stock |
 BL21/ipdC | Carries pET28a-ipdC | This work |
 BL21/kivD | Carries pET28a-kivD | This work |
 BL21/D289L | Carries pET28a-ipdC-D289L | This work |
 BL21/T290L | Carries pET28a-ipdC-T290L | This work |
 BL21/Q383M | Carries pET28a-ipdC-Q383M | This work |
 BL21/A387I | Carries pET28a-ipdC-A387I | This work |
 BL21/F388W | Carries pET28a-ipdC-F388W | This work |
 BL21/A387L | Carries pET28a-ipdC-A387L | This work |
 BL21/V542I | Carries pET28a-ipdC-V542I | This work |
 BL21/L546W | Carries pET28a-ipdC-L546W | This work |
 BL21/D289L + T290L | Carries pET28a-ipdC-D289L + T290L | This work |
 BL21/A387I + F388W | Carries pET28a-ipdC-A387I + F388W | This work |
Plasmids | ||
 pDK6 | Kanr, lacIQ, tac, 5.1 kb | [19] |
 pDK6-kivD | pDK6 carries kivD | [18] |
 pDK6-ipdC | pDK6 carries ipdC | [18] |
 pET28a | Vector carries N-terminal His Tag, Kanr, 5369 bp | Novagen® |
 pGEM-kivD | Vector holding kivD (L. lactis) | [20] |
 pMD18-T-ipdC | Ampr, carries ipdC | [18] |
 pET28a-kivD | pET28a carries kivD | This work |
 pET28a-ipdC | pET28a carries ipdC | This work |