Table 4 Strains used in this study
From: Engineering proton-coupled hexose uptake in Saccharomyces cerevisiae for improved ethanol yield
Strain name | Relevant genotype | Source |
|---|---|---|
CEN.PK113-7D | MATa URA3 HIS3 LEU2 TRP1 MAL2-8c SUC2 | Â |
Kluyveromyces lactis CBS 2359 | Wild type | CBS-KNAW |
Saccharomyces eubayanus CBS 12,357 | Wild type | CBS-KNAW |
Kluyveromyces marxianus CBS 6556 | Wild type | CBS-KNAW |
IMX1812 | MATa ura3-52 trp1-1 leu2-3,112 his3Δ can1::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ | [37] |
IMX2115 | MATa ura3-52 trp1-1 leu2-3,112 HIS3 can1::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ | This study |
IMX2125 | MATa ura3-52 trp1-1 LEU2 HIS3 can1Δ::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ | This study |
IMX2144 | MATa ura3-52 TRP1 LEU2 HIS3 can1::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ | This study |
IMZ756 | IMX2144 + pUDE432 (URA3 MAL11) | This study |
IMZ757 | IMX2144 + pUDE897 (URA3 KlFRT1) | This study |
IMZ759 | IMX2144 + pUDE898 (URA3 SeFSY1) | This study |
IMZ763 | IMX2144 + pUDE914 (URA3 HXT5) | This study |
IMZ767 | IMX2144 + pUDE920 (URA3 KmHGT1) | This study |
IMZ796 | IMX2144 + p426GPD (URA3) | This study |
IMS1058 | Single-colony isolate of anaerobically evolved IMZ756 | This study |
IMS1059 | Single-colony isolate of anaerobically evolved IMZ757 | This study |
IMS1060 | Single-colony isolate of anaerobically evolved IMZ759 | This study |
IMS1061 | Single-colony isolate of anaerobically evolved IMZ767 | This study |
IMK698 | MATa ura3-52 leu2-112 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP-kanMX-loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ | [41] |
IMX2572 | MATa ura3-52 leu2-112 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP-kanMX-loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 | This study |
IMK980 | MATa ura3-52 leu2-112 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 | This study |
IMK983 | MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 | This study |
IMK985 | MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ | This study |
IMK990 | MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ | This study |
IMK992 | MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ | This study |
IMK1003 | MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ | This study |
IMK1008 | MATa ura3-52 LEU2 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ | This study |
IMK1010 | MATa ura3-52 LEU2 HIS3 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ | This study |
IMX2719 | MATa ura3-52 LEU2 HIS3 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ sga1::SUC2 | This study |
IMZ783 | IMX2719 + pUDE1028 (URA3 KmHGT1 SeFSY1) | This study |
IMZ785 | IMX2719 + pUDE914 (URA3 HXT5) | This study |
IMS1214 | Single-colony isolate of anaerobically evolved IMZ783 | This study |
IMS1215 | Single-colony isolate of anaerobically evolved IMZ783 | This study |
IME666 | IMX2144 + pUDE1089 (URA3 KlFRT1T767C) | This study |
IME752 | IMX2719 + pUDE1221 (URA3 KmHGT1T1058C SeFSY1) | This study |