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Table 4 Strains used in this study

From: Engineering proton-coupled hexose uptake in Saccharomyces cerevisiae for improved ethanol yield

Strain name Relevant genotype Source
CEN.PK113-7D MATa URA3 HIS3 LEU2 TRP1 MAL2-8c SUC2  
Kluyveromyces lactis CBS 2359 Wild type CBS-KNAW
Saccharomyces eubayanus CBS 12,357 Wild type CBS-KNAW
Kluyveromyces marxianus CBS 6556 Wild type CBS-KNAW
IMX1812 MATa ura3-52 trp1-1 leu2-3,112 his3Δ can1::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ [37]
IMX2115 MATa ura3-52 trp1-1 leu2-3,112 HIS3 can1::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ This study
IMX2125 MATa ura3-52 trp1-1 LEU2 HIS3 can1Δ::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ This study
IMX2144 MATa ura3-52 TRP1 LEU2 HIS3 can1::Spcas9-natNT2 gal2Δ hxt4-1-5Δ hxt3-6–7::ars4 hxt8Δ hxt14Δ hxt2Δ hxt9Δ hxt10Δ hxt11Δ hxt12Δ hxt13Δ hxt15Δ hxt16Δ mph2Δ mph3Δ mal11Δ stl1Δ This study
IMZ756 IMX2144 + pUDE432 (URA3 MAL11) This study
IMZ757 IMX2144 + pUDE897 (URA3 KlFRT1) This study
IMZ759 IMX2144 + pUDE898 (URA3 SeFSY1) This study
IMZ763 IMX2144 + pUDE914 (URA3 HXT5) This study
IMZ767 IMX2144 + pUDE920 (URA3 KmHGT1) This study
IMZ796 IMX2144 + p426GPD (URA3) This study
IMS1058 Single-colony isolate of anaerobically evolved IMZ756 This study
IMS1059 Single-colony isolate of anaerobically evolved IMZ757 This study
IMS1060 Single-colony isolate of anaerobically evolved IMZ759 This study
IMS1061 Single-colony isolate of anaerobically evolved IMZ767 This study
IMK698 MATa ura3-52 leu2-112 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP-kanMX-loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ [41]
IMX2572 MATa ura3-52 leu2-112 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP-kanMX-loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 This study
IMK980 MATa ura3-52 leu2-112 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 This study
IMK983 MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 This study
IMK985 MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ This study
IMK990 MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ This study
IMK992 MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ This study
IMK1003 MATa ura3-52 leu2-112 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ This study
IMK1008 MATa ura3-52 LEU2 his3Δ MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ This study
IMK1010 MATa ura3-52 LEU2 HIS3 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ This study
IMX2719 MATa ura3-52 LEU2 HIS3 MAL2-8C mal11-mal12::loxP mal21-mal22::loxP mal31-mal32::loxP mph2/3::loxP mph2/3::loxP-hphNT1-loxP suc2::loxP ima1Δ ima2Δ ima3Δ ima4Δ ima5Δ can1::cas9-natNT2 hxt8Δ hxt14Δ gal2Δ hxt4Δ hxt1Δ hxt5Δ hxt3Δ hxt6Δ hxt7Δ hxt13Δ hxt15Δ hxt16Δ hxt2Δ hxt10Δ hxt9Δ hxt11Δ hxt12Δ stl1Δ sga1::SUC2 This study
IMZ783 IMX2719 + pUDE1028 (URA3 KmHGT1 SeFSY1) This study
IMZ785 IMX2719 + pUDE914 (URA3 HXT5) This study
IMS1214 Single-colony isolate of anaerobically evolved IMZ783 This study
IMS1215 Single-colony isolate of anaerobically evolved IMZ783 This study
IME666 IMX2144 + pUDE1089 (URA3 KlFRT1T767C) This study
IME752 IMX2719 + pUDE1221 (URA3 KmHGT1T1058C SeFSY1) This study
  1. Unless specified otherwise, these concern S. cerevisiae strains