Fig. 1

H₂ production from formate by resting T. kivui cells in presence or absence of metabolic inhibitors. Resting cells (0.6 mg mL⁻¹) were added to preheated (60 °C) imidazole buffer (50 mM imidazole, 20 mM MgSO₄, 20 mM KCl, 2 mM DTE, 4 µM resazurin, pH 7) under a N₂ atmosphere. a The reaction was started by addition of 150 mM of sodium formate. H₂ (black squares) and formate concentrations (black triangles up) are plotted against time. b 30 µM DCCD, TCS or ETH2120 or 20 µL ethanol was added to the serum bottles 10 min before the reaction was started by adding 150 mM of sodium, potassium or ammonium formate as indicated. The specific hydrogen production rate was calculated based on the first 15 min after start of the reaction. All data points are mean ± SD, N = 2