Fig. 3From: Formate-driven H2 production by whole cells of Thermoanaerobacter kivuiFormate- and cell density-dependent H2 production at optimal pH and temperature. Resting cells were added to preheated (70 °C) imidazole buffer (50 mM imidazole, 20 mM MgSO4, 20 mM KCl, 2 mM DTE, 4 µM resazurin, pH 7) under N2 atmosphere. The reaction was started by adding sodium formate. Black squares, 0.3 mg mL−1 and 300 mM sodium formate; black triangles down, 4 mg mL−1 and 600 mM sodium formate. All data points are mean ± SD, N = 2Back to article page