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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Formate-driven H2 production by whole cells of Thermoanaerobacter kivui

Fig. 5

Formate-driven H2 production in a batch-operated stirred-tank bioreactor without pH control [Batch 2]. The bioreactor contained imidazole buffer (50 mM imidazole, 20 mM KCl, 2 mM DTE, pH 7.0) at a temperature of 60 °C. The stirrer speed was set at 400 rpm and a continuous gas flow rate of 50 mL min−1 with 100% N2 was applied. 600 mM of sodium formate was added prior to the start of the experiment. The reaction was started by adding resting cells to a final total cell protein concentration of 0.6 mg mL−1 into the bioreactor. The pH value was not controlled. a The formate consumption and pH are shown over time. b Gas evolution of H2 and CO2 during the experiment. c Optical density and total cell protein concentration in the bioreactor during the whole process time. Black triangles up, formate; black circles, pH value; black diamonds, H2; black triangles down, CO2; empty triangles up, total cell protein concentration; empty diamonds, optical density at 600 nm. All data points are mean ± SD, N = 2

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