Fig. 5From: Inhibitory effect of lignin on the hydrolysis of xylan by thermophilic and thermolabile GH11 xylanasesXylanase distribution after incubation with enzymatic hydrolysis residue (EnzHR) lignin. Distribution of xylanases a TrXyn1, b TrXyn2, c Xyl40 and d Xyl40-CD to the supernatant (free enzyme) and solid (lignin-bound enzyme) fractions after incubating 10 µg/ml xylanase with 10 mg/ml EnzHR lignin from isolated from steam pretreated spruce. Enzyme incubated without lignin was used as a control sample. These are same samples as are presented in Fig. 4. Xylanase band is indicated with a triangle and band intensities compared to control as presented as % of control sample at 3 h (% data not shown for Xyl40). A band for BSA, which was added after incubation with lignin, is visible in each sample above the 50 kDa standard. Each xylanase was run on their own gel, but for presentation purposes for b, bands were cut to remove unnecessary lanes and indicated as a gap in the imageBack to article page