Fig. 1

Cellulase activity measurement using fluorescein di-beta-D-cellobioside (FCB). a Mechanism of fluorescence-based cellulase activity measurement. Green fluorescent compound fluorescein can be released from FCB by cleavage of the β-1,4-D-glycosidic bonds under cellulase treatment. b Fluorescence-based cellulase activity assay using droplet (solid line) and MTP (dashed line) formats