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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

Fig. 2

Effect of methanol on fluorescein leakage in droplets. a Histogram of nonfluorescent droplets only containing BMMY medium with 1% methanol. b Histogram of fluorescent droplets generated from 40 h reaction solution of cellulase in BMMY medium with 1% methanol and 20 μM fluorogenic substrate FCB. c Histogram of a binary mixture of nonfluorescent and fluorescent droplets at a 10:1 mixing ratio. d A typical recording of fluorescence signals when detecting the binary droplet mixture. e Fluorescence microscopy image of the binary droplet mixture. Droplets were incubated at 30 °C for 48 h. All histograms show approximately up to 10,000 droplets

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