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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris

Fig. 4

Workflow of the droplet-based microfluidic screening. a Droplet generation. Cells were encapsulated in microdroplets together with the substrate FCB using an appropriate cell to droplet ratio. b Droplet incubation. The emulsion was incubated off-chip in a syringe to allow cell growth, cellulase expression, and fluorescence signal development. c Droplet reinjection. After incubation, the emulsion droplets were reinjected into a sorting device. d Droplet fluorescence detection. The fluorescence signal of each droplet was detected and analyzed by the optical setup. e Droplet sorting. Droplets were sorted based on resulting fluorescence using dielectrophoresis

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