Fig. 5

qRT-PCR analysis of the expression of key genes in the TAG biosynthesis and membrane lipid turnover in the transformants at exponential phase (day 2) under standard growth conditions. The relative expression level of diacylglycerol acyltransferases (DGAT) (a), phospholipid:diacylglycerol acyltransferase 1 (PDAT1) (b), and plastid galactoglycerolipid degradation 1 (PGD1) (c). The housekeeping gene RACK1 (Cre06.g278222) was used as the internal standard. Values are the relative expression level compared with parental line UVM4. Error bars represent standard deviations based on three biological replicates (independent cultures) with three technical replicates each. Asterisks indicate statistically significant changes compared to the parental line UVM4 by paired-sample Student’s t test (*P ≤ 0.05; **P ≤ 0.01)