Fig. 1

Combinatorial power of VersaTile. The docking enzyme assembly system allows the construction of monocatalytic and bicatalytic docking enzymes. The position of the dockerin and enzymes can be chosen freely. Monocatalytic docking enzymes can be constructed with or without a linker separating the dockerin and enzymatically active module. The docking enzyme tile repository allows the construction of 3578 GM-degrading docking enzymes (top panel). The scaffoldin assembly system allows the construction of monovalent, bivalent, trivalent, tetravalent and pentavalent scaffoldins. The scaffoldin tile repository allows the construction of 43,833 scaffoldins (middle panel). The combination of both assembly systems allows us to consider 6 × 10¹⁷ different GM-degrading designer cellulosomes (bottom panel). The number of proteins that can be constructed was calculated using the number of tiles present in the tile repository and shown in Tables 1 and 2