Fig. 3
From: Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study
Influence of docking enzyme architecture on the activity of docking enzymes. A. Influence of dockerin position on the activity of T. fusca mannosidase docking enzymes. The activity of Tf-Manno_Doc-CtI (construct nr. 33) and Doc-CtI_Tf-Manno (construct nr. 34) are shown. Reaction mixtures were composed of 1 mL 2.5 mM pNP-β-mannose and 3 pmol enzyme. B. Influence of dockerin position and modular arrangement on the activity of the C. cellulolyticum galactosidase docking enzymes. The activity of Cc-Aga-Li_Doc-CtI (construct nr. 42), Doc-CtI_Cc-Aga-Li (construct nr. 43) and Cc-Aga-CBM_Doc-CtI (construct nr. 44) are shown. Reaction mixtures were composed of 1 mL 2.5 mM pNP-α-galactose and 3 pmol enzyme. Enzymatic activity was determined quantitatively by measuring the released pNP. Slopes were converted to enzymatic activity (U/nmol). Each bar represents the mean ± SD of three replicates. *p < 0.05, Student’s t-test