Fig. 6
From: Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study
Released reducing sugars, mannose and galactose when different docking enzymes are incorporated in the complex. The activity of complexes composed of Ct-CBM3_Coh-Ac_Coh-Bc_Coh-CtI (construct nr. 135), Tf-Manna_Li-G_Doc-Ac (construct nr. 26, white), Tf-Manno_Doc-Bc (construct nr. 39, light grey) and/or Cj-Aga_Li-D_Doc-CtI (construct nr. 63, dark grey) was tested. Reaction mixtures were composed of 1 mL 0.5% GM and 10 pmol designer cellulosome. Enzymatic activity was determined quantitatively by measuring the released reducing sugars, mannose and galactose over time. An ANOVA followed by a post hoc Tukey test revealed a significant variation among the tested complexes. *Detected below limit of detection