Fig. 7
From: Combinatorial assembly and optimisation of designer cellulosomes: a galactomannan case study
Release of reducing sugars, mannose and galactose monomers for six cellulosomal variants. By employing different scaffoldin variants, enzymes were located at different positions in the complex. All tested complexes contained Tf-Manna_Li-G_Doc-Ac (construct nr. 26, white), Tf-Manno_Doc-Bc (construct nr. 39, light grey) and Cj-Aga_Li-D_Doc-CtI (construct nr. 63, dark grey) and one of the following scaffoldins: Ct-CBM3_Coh-Ac_Coh-Bc_Coh-CtI (construct nr. 135), Ct-CBM3_Coh-Ac_Coh-CtI_Coh-Bc (construct nr. 136), Ct-CBM3_Coh-Bc_Coh-Ac_Coh-CtI (construct nr. 137), Ct-CBM3_Coh-CtI_Coh-Ac_Coh-Bc (construct nr. 138), Ct-CBM3_Coh-Bc_Coh-CtI_Coh-Ac (construct nr. 139) or Ct-CBM3_Coh-CtI_Coh-Bc_Coh-Ac (construct nr. 140). Reaction mixtures were composed of 1 mL 0.5% GM and 10 pmol designer cellulosome. Enzymatic activity was determined quantitatively by measuring the released reducing sugars, mannose and galactose over time. An ANOVA followed by a post hoc Tukey test revealed a significant variation among the tested complexes