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Fig. 1 | Biotechnology for Biofuels and Bioproducts

Fig. 1

From: Engineering the oleaginous yeast Candida tropicalis for α-humulene overproduction

Fig. 1

Biosynthesis of α-humulene in C. tropicalis. a Scheme showing the α-humulene biosynthesis pathway in C. tropicalis. ERG10, acetoacetyl-CoA thiolase; ERG13, hydroxymethylglutaryl-CoA synthase; tHMRG, truncated hydroxymethylglutaryl-CoA reductase; NADH-HMGR, NADH-dependent HMG-CoA reductase from Silicibacter pomeroyi; ERG12, mevalonate kinase; ERG8, phosphomevalonate kinase; ERG19, mevalonate diphosphate decarboxylase; IDI1, isopentenyl diphosphate isomerase; ERG20, geranyl/farnesyl diphosphate synthase; ZSS1, α-humulene synthase; IPP, isopentenyl diphosphate; DMAPP, dimethylallyl diphosphate; GPP, geranyl diphosphate; FPP, farnesyl diphosphate. b α-humulene production by C. tropicalis expressing ZSS1 in the cytoplasm (HC01 and HC02) and in peroxisomes (HP01 and HP02). Results are means ± standard deviations of biological triplicates. c Fluorescence microscopy of C. tropicalis 01 co-expressed the ZSS1-GFP-PTS1 fusion protein and the peroxisome marker PEX3-mScarlet

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