Fig. 6

Acetate secretion by S. cerevisiae CEN.PK 2-1D (wild type) (◇) and aqr1∆ (◼), tpo2∆tpo3∆ (●) and aqr1∆tpo2∆tpo3∆ (▲) mutants. Cells were loaded with 50 mM [1-¹⁴C-] acetate for 15 min and subsequently diluted into a medium with acetate, and the intracellular [1-¹⁴C] acetate was measured in time by filtration as described in the Materials and methods section. Data are shown as average and standard deviation of biological duplicate and expressed in nmol acetate/OD₆₀₀. One-way ANOVA analysis and Tukey’s honest significant difference test were used to analyze the statistical difference between mutants and wild type at the same time point. *P ≤ 0.05