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Table 1 Experimental SAXS parameters derived from the scattering curves of the various scaffoldins, components and complexes

From: Mapping the deformability of natural and designed cellulosomes in solution

Construct

Organism

Rg (Ã…)

Dmax (Ã…)

Mw (kDa)

χ2 of fit

Scaffoldins and components

 ScaA

R. champanellensis

60.4 ± 0.2

282 ± 11

68.7

3.1

 X module of ScaA

R. champanellensis

21.6 ± 0.1

91 ± 5

23.2

3.7

 ScaH

R. champanellensis

55.8 ± 0.2

230 ± 15

55.1

3.1

 SGNH module of ScaH

R. champanellensis

27.1 ± 0.6

103 ± 5

29.3

2.6

 ScaK

R. champanellensis

44.9 ± 0.2

184 ± 6

52.3

5.3

 Scaf20L

Chimeric scaffoldin: CBM and cohesin from C. thermocellum, cohesins from A. cellulolyticus and B. cellulosolvens

66.3 ± 0.4

262 ± 10

75.3

1.1

Enzymes

 Cel48S-t

Wild-type GH48S and dockerin from C. thermocellum

34.2 ± 0.1

148 ± 4

81.6

1.3

 Cel8A-b

Chimeric enzyme: GH8 from C. thermocellum, dockerin from B. cellulosolvens

29.9 ± 0.3

118 ± 3

51.6

8.3

 Cel9A-r

Wild-type enzyme, GH9-CBM3c and dockerin from R. champanellensis

35.2 ± 0.1

110 ± 3

91.8

5.5

DC complexes

 Scaf20L + Cel8A-b

Chimeric scaffoldin and chimaeric enzyme

64.3 ± 0.3

251 ± 8

126.9

1.1

 Scaf20L + Cel9R-a + Cel8A-b + Cel48S-t

Chimeric scaffoldin and chimaeric and wild-type enzymes from C. thermocellum bearing dockerins that match the cohesins of Scaf20L

90.9 ± 0.5

305 ± 15

300.3

1.3

CipA and complex

 CipA

Wild-type scaffoldin from C. thermocellum

157 ± 1.6

530 ± 20

198.1

ND

 CipA-∆XD + Cel8A-t

Truncated scaffoldin and wild-type enzyme from C. thermocellum

151 ± 1.7

497 ± 18

632.2

ND

 CipA + Cel8A-t

Wild-type scaffoldin and wild-type enzyme from C. thermocellum

170.1 ± 1.2

575 ± 20

651.7

ND