From: Mapping the deformability of natural and designed cellulosomes in solution
Construct | Organism | Rg (Å) | Dmax (Å) | Mw (kDa) | χ2 of fit |
---|---|---|---|---|---|
Scaffoldins and components | |||||
 ScaA | R. champanellensis | 60.4 ± 0.2 | 282 ± 11 | 68.7 | 3.1 |
 X module of ScaA | R. champanellensis | 21.6 ± 0.1 | 91 ± 5 | 23.2 | 3.7 |
 ScaH | R. champanellensis | 55.8 ± 0.2 | 230 ± 15 | 55.1 | 3.1 |
 SGNH module of ScaH | R. champanellensis | 27.1 ± 0.6 | 103 ± 5 | 29.3 | 2.6 |
 ScaK | R. champanellensis | 44.9 ± 0.2 | 184 ± 6 | 52.3 | 5.3 |
 Scaf20L | Chimeric scaffoldin: CBM and cohesin from C. thermocellum, cohesins from A. cellulolyticus and B. cellulosolvens | 66.3 ± 0.4 | 262 ± 10 | 75.3 | 1.1 |
Enzymes | |||||
 Cel48S-t | Wild-type GH48S and dockerin from C. thermocellum | 34.2 ± 0.1 | 148 ± 4 | 81.6 | 1.3 |
 Cel8A-b | Chimeric enzyme: GH8 from C. thermocellum, dockerin from B. cellulosolvens | 29.9 ± 0.3 | 118 ± 3 | 51.6 | 8.3 |
 Cel9A-r | Wild-type enzyme, GH9-CBM3c and dockerin from R. champanellensis | 35.2 ± 0.1 | 110 ± 3 | 91.8 | 5.5 |
DC complexes | |||||
 Scaf20L + Cel8A-b | Chimeric scaffoldin and chimaeric enzyme | 64.3 ± 0.3 | 251 ± 8 | 126.9 | 1.1 |
 Scaf20L + Cel9R-a + Cel8A-b + Cel48S-t | Chimeric scaffoldin and chimaeric and wild-type enzymes from C. thermocellum bearing dockerins that match the cohesins of Scaf20L | 90.9 ± 0.5 | 305 ± 15 | 300.3 | 1.3 |
CipA and complex | |||||
 CipA | Wild-type scaffoldin from C. thermocellum | 157 ± 1.6 | 530 ± 20 | 198.1 | ND |
 CipA-∆XD + Cel8A-t | Truncated scaffoldin and wild-type enzyme from C. thermocellum | 151 ± 1.7 | 497 ± 18 | 632.2 | ND |
 CipA + Cel8A-t | Wild-type scaffoldin and wild-type enzyme from C. thermocellum | 170.1 ± 1.2 | 575 ± 20 | 651.7 | ND |