Fig. 2

HFA content of pCam5 vector transformants. a T-DNA structure of pCam5 vector of five castor gene sets (promoter–gene–terminator). Set1, Phas-FAH12-Nos (2986 bp); Set2, FAE1-RcPDAT1-2-PinII (3324 bp); Set3, Napin-RcLPCAT-Nos (2794 bp); Set4, Phas-RcDGAT2-PinII (2989 bp); Set5, FAE1-RcPDCT-Nos (2050 bp). Each set was sequentially cloned into pCambia3300 vector. The restriction enzyme sites used for cloning are marked on the vector. The size of entire five expression sets is 14.3 kb, and the vector backbone of pCambia 3300 is 8.4 kb, resulting in pCam5 vector in size of 22.7 kb. Phas, phaseolin promoter (1500 bp); FAE1, Fatty acid elongase 1 promoter (946 bp); Napin, napin promoter (1152 bp); Nos, Nos terminator (255 bp); PinII, PinII terminator (406 bp); FAH12 (1164 bp), DGAT2 (1023 bp), RcPDAT1-2 (1,983 bp), LPCAT (1398 bp), PDCT (838 bp), LB, Left border; RB, Right Border. b HFA content in T2 and T4 seeds generations of pCam5 transformants