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Fig. 1 | Biotechnology for Biofuels and Bioproducts

Fig. 1

From: Characterization of a novel thermophilic metagenomic GH5 endoglucanase heterologously expressed in Escherichia coli and Saccharomyces cerevisiae

Fig. 1

Structural protein alignment of three models for Cel776 generated with SWISS-MODEL. A Model 1 was constructed using as template 3rjy.1.A, the endoglucanase from Fervidobacterium nodosum (FnCel5A) in complex with substrate alpha-d-glucopyranose (green). Model 2 was constructed using as template 3azr.1.A, an inactive mutant of the endoglucanase from Thermotoga maritima [TmCel5A (E253A)] in complex with cellobiose (yellow). Model 3 was constructed using as template 4u5i.1.A, an inactive mutant endoglucanase from Clostridium thermocellum [CtCel5E (E314A)] in complex with xylobiose (light blue). B Structural alignment of the three generated models highlighting the secondary structure (alpha helices in blue, beta sheets in green). C Structural alignment of the three generated models highlighting the indels. Substrates are represented in ball-and-stick model

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