Fig. 3

SDS–PAGE and western blot for Cel776. A SDS–PAGE of the purification process for the Cel776Ec gene product. M: NZYColour protein marker II (NZYTech, Portugal) 2.5 µL; CE: Crude Extract 9 µL; FT: flow-through from the column load and wash steps 9 µL; E10: Elution fraction at 10% elution buffer concentration 18 µL; E50: Elution fraction at 50% elution buffer concentration 18 µL; E100: Elution fraction at 100% elution buffer concentration, 18 µL. Black arrows mark the band corresponding to the Cel776Ec protein. B Western blot of the purification steps for the Cel776Ec gene product. Lanes are named as in A