Table 1 Comparison of enzymatic parameters of thermophilic GH5 family endoglucanases with Cel776Ec and Cel776Sc

FnCel5A

Fervidobacterium nodosum

E. coli

80–83 °C

pH 5.0–5.5

Half-life of 48 h at 80 °C

No

440 U/mg

NM

[30]

TmCel5A

Thermotoga maritima

E. coli

90 °C

pH 6.6

Stable at 85 °C

No

80 U/mg

Km = 0.24 mM with pnp-B-D-cellopentaoside

[31]

CtCel5E

Clostridium thermocellum

E. coli

50 °C

pH 5.0

Not determined

Yes

736.2 ± 12.8 U

Km = 2.1 ± 0.2 (mg/mL); kcat = 1564.0 ± 69.1 min⁻¹

[28]

Cel776Ec

Hot Spring Metagenome

E. coli

80 °C

pH 5.0

Half-life of 3.55 h at 80 °C

Yes

2.12 U/mg

NM

This work

Cel776Sc

Hot Spring Metagenome

S. cerevisiae

80 °C

pH 5.0

60% activity after 1 h at 70 °C

NM

NM

Km = 0.1716 µg µL⁻¹; Vmax 0.6686 U µL⁻¹

This work