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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Engineering astaxanthin accumulation reduces photoinhibition and increases biomass productivity under high light in Chlamydomonas reinhardtii

Fig. 4

Photooxidation of bkt5 and UVM4 under light stress. A UVM4 (black) and bkt5 (red) cell suspensions were treated with strong white light (14,000 μmol photons/m2/s, 20 °C) and chlorophyll bleaching was evaluated by measuring the absorption area in the region 620–740 nm. B Oxygen evolution of UVM4 (black) and bkt5 (red) cells illuminated with cycles of 5 minutes of white light at 6000 μmol photons m−2 s−1(yellow bar) and three minutes of dark (black bar). C Oxygen evolution during continuous illumination of UVM4 (black) and bkt5 (red) with white light at 6000 μmol photons m−2 s−1. D Singlet oxygen production of UVM4 (black) and bkt5 (red) cell suspension, estimated by measuring Singlet Oxygen Sensor Green (SOSG) fluorescence emission (excitation 480 nm, emission 510–540 nm). Cells were illuminated with 2000 μmol photons m−2 s−1red light at 20 °C. Error bars are reported as standard deviation (n = 4). Values significantly different comparing bkt5 with UVM4 are reported with * (Student’s t test, P < 0.05)

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