Circadian rhythm promotes the biomass and amylose hyperaccumulation by mixotrophic cultivation of marine microalga Platymonas helgolandica

Table 1 The kinetic parameters for growth and starch accumulation of P. helgolandica cultures under different cultivation modes (mean ± SD, n = 3)

	Autotrophy	Mixotrophy	Mixotrophy under circadian cycle			Heterotrophy
	24:0 (-Glc)	24:0	18:6	12:12	6:18	0:24
X1_max (g_TS L⁻¹)	0.34 ± 0.01	0.91 ± 0.02	2.71 ± 0.17	3.20 ± 0.09	3.88 ± 0.16	1.65 ± 0.02
X2_max (g_Am L⁻¹)	0.17 ± 0.00	0.44 ± 0.01	1.64 ± 0.08	2.09 ± 0.06	2.53 ± 0.11	0.83 ± 0.01
X3_max (g_DW L⁻¹)	1.81 ± 0.07	3.13 ± 0.01	6.14 ± 0.12	6.53 ± 0.03	6.35 ± 0.17	2.67 ± 0.11
Y (g_Am g_Glc⁻¹)	/	0.05 ± 0.01	0.18 ± 0.00	0.23 ± 0.01	0.28 ± 0.01	0.09 ± 0.00
μ (D⁻¹)	0.07 ± 0.01	0.85 ± 0.01	1.30 ± 0.04	1.31 ± 0.01	1.43 ± 0.02	0.76 ± 0.03
Q_X1max (g_TS·L⁻¹ D⁻¹)	0.03 ± 0.00	0.08 ± 0.00	0.24 ± 0.02	0.28 ± 0.05	0.40 ± 0.08	0.14 ± 0.02
Q_X2max (g_Am·L⁻¹ D⁻¹)	0.01 ± 0.00	0.04 ± 0.00	0.17 ± 0.02	0.19 ± 0.05	0.25 ± 0.06	0.07 ± 0.01

X1—total starch, X2—amylose, X3—dry weight, Y—yield of amylose on glucose, μ—growth rate, Q_X1max—production rate of total starch, Q_X2max—production rate of amylose

ISSN: 2731-3654