Fig. 2

Analysis of the overexpression of Hmg1 isoforms for limonene production in A. gossypii. A Domain organization of the yeast Hmg1 protein (green); the A. gossypii isoforms (Hmg1, tHmg1-1 and tHmg1-2) are depicted (orange) for comparison with the functional domains of the yeast protein. B Limonene production of the A. gossypii engineered strains with different HMG1 isoforms and either containing or lacking the PKT pathway. The parental strain contained overexpression modules for the XR-XDH-XK pathway and the C. limon LS. The results are the means of two independent experiments performed in duplicate. The cultures were performed in flasks for 72 h with 0.5% glucose plus 2% xylose as the carbon sources