Fig. 5
From: Metabolic engineering of Ashbya gossypii for limonene production from xylose
Synthetic orthogonal pathway for the production of limonene in A. gossypii. The overexpression of the tNDPS1 gene from S. lycopersicum creates a metabolic bypass of ERG20 (orthogonal pathway). The limonene synthase (LS) can utilize both NPP and GPP. The tNDPS1 gene was overexpressed using the strong promoter PTSA1 in the parental strain A1206. The production of limonene in the engineered strain was measured in flask cultures grown for 72 h with 0.5% glucose plus 2% xylose as the carbon sources. The results are the means of two independent experiments performed in duplicate