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Fig. 7 | Biotechnology for Biofuels and Bioproducts

Fig. 7

From: Metabolic engineering of Ashbya gossypii for limonene production from xylose

Fig. 7

Optimization of the production of limonene in A. gossypii. A The genes ERG12, ERG8 and ERG19 were overexpressed using the specified strong promoters (PSED1 or PTSA1). The parental strain was A1238. B The allele erg20F95W was combined with the ERG12 overexpression in the A1308 strain. The mutant erg20F95W was expressed either under a strong promoter (PGPD1-erg20F95W) or from its native promoter (erg20F95W). C Growth phenotype of engineered strains expressing different alleles of ERG20 in the A1308 parental strain. A1373 contains PGPD1-ERG20; A1372 contains PGPD1-erg20F95W; A1388 contains PERG20-erg20F95W. The blue circle represents the growth of the wild-type ERG20. Solid MA2 plates were cultured for 48 h. D The strain A1308 was cultured at the indicated time points. Limonene titers and biomass (dry cell weight) were calculated. The production of limonene in the engineered strains was measured in flask cultures grown for 72 h (except for D) with 0.5% glucose plus 2% xylose as the carbon sources. The results are the means of two independent experiments performed in duplicate

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