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Table 1 Limonene titer of selected engineered strains on A. gossypii

From: Metabolic engineering of Ashbya gossypii for limonene production from xylose

Strain number Modificationsa Limonene titer (mg/L) Limonene titer (mg/g)
A1206 XR-XDH-XH + PKT + tLS + HMG1 1.38 ± 0.15 0.15 ± 0.01
A1224 XR-XDH-XH + PKT + tLS + HMG1 + IDI1 2.27 ± 0.16 0.33 ± 0.06
A1242 XR-XDH-XH + PKT + tLS + HMG1 + ERG20 0.17 ± 0.2 0.03 ± 0.03
A1304 XR-XDH-XH + PKT + tLS + HMG1 + erg20F95W 2.9 ± 0.25 0.45 ± 0.01
A1251 XR-XDH-XH + PKT + tLS + HMG1 + erg20N126W 2.34 ± 0.13 0.43 ± 0.01
A1238 XR-XDH-XH + PKT + tLS + HMG1 + tNDPS1 33.6 ± 7.8 2.6 ± 1.17
A1308 XR-XDH-XH + PKT + tLS + HMG1 + tNDPS1 + ERG12 (72 h) 173.33 ± 18.49 36.89 ± 4.05
A1372 XR-XDH-XH + PKT + tLS + HMG1 + tNDPS1 + ERG12 + erg20F95W 96 ± 15.98 19.55 ± 3.25
A1388 XR-XDH-XH + PKT + tLS + HMG1 + tNDPS1 + ERG12 + erg20F95W (no overexpression) 143.3 ± 8.76 60.72 ± 3.71
A1308 XR-XDH-XH + PKT + tLS + HMG1 + tNDPS1 + ERG12 (96 h) 336.4 ± 13.86 81.88 ± 3.93
  1. aBold indicates the gene that was overexpressed/manipulated in each engineered strain