From: Metabolic engineering of Ashbya gossypii for limonene production from xylose
Organism | Carbon source; culture mode | Limonene synthase | Relevant modifications | Titer (mg/L) | Refs. |
---|---|---|---|---|---|
E. coli | Glycerol; bioreactor; fed-batch | Mentha spicata, codon-optimized, truncated | Plasmid for gene overexpression of: atoB and idi from E. coli; ERG13, tHMG1, ERG12, ERG8 and ERG19 from S. cerevisiae; and tGPPS from Abies grandis | 3630 | [43] |
E. coli | Glucose; shake flask; fed-batch | M. spicata, codon-optimized | MvaE and MvaSA110G from Enterococcus faecalis with synthetic RBS; MVK (ERG12) from Methanosarcina mazei; ERG8, ERG19 and IDI1 from S. cerevisiae; and tNDPS1 from S. lycopersicum | 1290 | [44] |
E. coli | Glycerol; bioreactor; fed-batch | M. spicata, codon-optimized, truncated | atoB from E. coli; ERG13, tHMG1, ERG12, ERG8, ERG19 and IDI1 from S. cerevisiae; and tGPPS from A. grandis | 1350 | [45] |
S. cerevisiae | Glucose and ethanol; shake flask, fed-batch | C. limon, codon-optimized, truncated | tHMG1, IDI1, tNDPS1 from S. lycopersicum; and ERG20F96W−N127W | 917.7 | [32] |
S. cerevisiae | Galactose:raffinose (2:1); shake flask; semi-batch | C. limon, mutant H570Y | HMG2K6R, gene fusion ERG20N127W-SlNDPS1 | 166 | [34] |
Y. lipolytica | Glucose; shake flask | A. rugosa, codon-optimized, truncated | HMG1, ERG12, and tNDPS1 from S. lycopersicum | 23.56 | [28] |
Y. lipolytica | Glycerol and citrate; bioreactor; fed-batch | Agastache rugosa, codon-optimized, truncated | HMG1, ERG12, tNDPS1 from S. lycopersicum | 165.3 | [46] |
Y. lipolytica | Glucose; bioreactor; fed-batch | C. limon or M. spicata | HMG1. Optimization of fermentation conditions | D-limonene:11.7; L-limonene:11.08 | [26] |