Fig. 4

SpGAT1 directly bound to promoters of key genes in the pyruvate bypass pathway and the glyoxylate shunt pathway to promote lipid accumulation. a Expression profiles of PDC, ACS, ICL1, ICL2, ACAT2, and ACAT1 of WT, OE::gat1, and \(\Delta gat1\) in the B2:3 group. b Expression profiles of PDC, ACS, ICL1, ICL2, ACAT2, and ACAT1 of WT, OE::gat1, and \(\Delta gat1\) in the B80:3 group. PDC, encoding pyruvate decarboxylase; ACS, encoding acetyl-CoA synthetase; ICL1 and ICL2, encoding isocitrate lyase 1 and 2; ACAT1, encoding acetyl-CoA acyltransferase 1; ACAT2, encoding acetyl-CoA acyltransferase 2. c Yeast one-hybrid assays to detect whether SpGAT1 bound to promoters of PDC, ACS, ICL1, ICL2, ACAT2, and ACAT1. Y1H Gold [pAbAi-gene promoter + pGADT7] is the negative control, and Y1H Gold [pAbAi-gene promoter + pGADT7-SpGAT1] is the experiment group. Data are presented as the means ( SE, \(n = 3\)). ns: no significant difference, *\(p < 0.01\), **\(p < 0.001\), ***\(p < 0.0001\)