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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: GATA-type transcriptional factor SpGAT1 interacts with SpMIG1 and promotes lipid accumulation in the oleaginous yeast \(Saitozyma \ podzolica\) zwy-2-3

Fig. 5

SpGAT1 interacted with SpMIG1 and these two genes had a positive regulation relationship. a Expression profiles of SpMIG1 in WT, OE::gat1, and \(\Delta gat1\) in the B2:3 and B80:3 groups. b Yeast two-hybrid assays to detect whether SpGAT1 had an interaction with SpMIG1. Y2H Gold [pGBKT7+ pGADT7-SpGAT1] and Y2H Gold [pGBKT7-SpMIG1+pGADT7] are negative control, Y2H Gold [pGBKT7-SpMIG1+pGADT7-SpGAT1] is experiment group. DDO: SD/-Leu/-Trp, QDO: SD/-Leu/-Trp/-His/-Ade, 3-AT: 3-amino-1,2,4-triazole. Data are presented as the means ( SE, \(n = 3\)). ns: no significant difference, *\(p < 0.01\), **\(p < 0.001\), ***\(p < 0.0001\)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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