Fig. 4
From: Toward bioproduction of oxo chemicals from C1 feedstocks using isobutyraldehyde as an example
In vitro framework converting C1 feedstocks to isobutyraldehyde and other chemicals. A Design of the cell-free system converting CO2 to isobutyraldehyde. The proposed in vitro pathway for isobutyraldehyde production was assembled using two parts. The first one converting CO2 to D-glyceraldehyde-3-phosphate (GAP) was generated based on the first two modules of ASAP [13] and was indicated in grey color. The second one converting 3-phosphoglycerate to isobutyraldehyde was indicated in pink color. Metabolite: Dihydroxyacetone (DHA); Dihydroxyacetone phosphate (DHAP); Glyceraldehyde-3-phosphate (GAP); 1,3-Biphosphoglycerate (1,3PG); 3-Phosphoglycerate (3PG); 2-Phosphoglycerate (2PG); Phosphoenolpyruvate (PEP); 2-acetolactate (2-AL); 2,3-Dihydroxy-isovalerate (DHKIV); 2-Ketoisovalerate (KIV); Isobutyraldehyde (IBAL). Enzymes: alcohol oxidase (aox); formolase (fls); dihydroxyacetone kinase (dak); triosephosphate isomerase (tpi). B Cell-free framework for producing a wide array of chemicals. Similar to the in vitro system of isobutyraldehyde, cell lysates with pathway-specific enzymes or the designed cell-free protein synthesis (CFPS) system can be employed for production of various chemicals by assembling with the first two modules of ASAP