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Fig. 6 | Biotechnology for Biofuels and Bioproducts

Fig. 6

From: In silico-guided metabolic engineering of Bacillus subtilis for efficient biosynthesis of purine nucleosides by blocking the key backflow nodes

Fig. 6

Construction of the general purine chassis bacterium by in silico-guided metabolic engineering strategy. a Schematic diagram for in silico-guided metabolic engineering strategy. b The retention times of purine nucleosides, bases, and nucleosides by HPLC. c Determination of purine nucleosides metabolites synthesized in the general purine chassis strain. All error bars indicate ± SD, n = 3. A value of P less than 0.05 was regarded to be a significant difference with the W168 strain using the T-test (*, P < 0.05; **, P < 0.01)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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