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Fig. 7 | Biotechnology for Biofuels and Bioproducts

Fig. 7

From: Lysophosphatidic acid acyltransferase 2 and 5 commonly, but differently, promote seed oil accumulation in Brassica napus

Fig. 7

Genes involved in the TAG metabolic pathway affected by BnLPAT2 and BnLPAT5 in rapeseed. a Differential gene expression involved in TAG biosynthesis in the overexpression, knockdown, and knockout lines of BnLPAT2 and BnLPAT5 at three developmental stages. The expression pattern of each DEG is shown by three grids, with the first, second, and third grids from left to right representing the relative log2 (FC) of the overexpression, RNAi, and Cas9 lines, respectively. Up-regulated genes are in red, and down-regulated genes are in blue. The color indicates the value of log2 (FC). b Differential gene expression involved in TAG biosynthesis in LP2 and LP5 at three different seed developmental stages. The expression pattern of each DEG is shown by three grids within two rows, with the first, second, and third grids from left to right representing the relative log2 (FC) at 14, 26, and 38 DAF, respectively, and the two rows from the top to the bottom representing the relative log2 (FC) in LP2 and LP5 lines. Glycerolipid classes showing increases in LP5 lines are highlighted with a blue background and those showing increases in LP2 lines are highlighted with a pink background. c Proposed model of oil accumulation in B. napus under regulation by BnLPAT2&5. The left part of the double dotted line represents the pathways that are mainly involved in the overexpression lines, whereas the right part represents the pathways that are mainly involved in the knockdown and knockout lines. Green lines represent the enhanced pathways in LP2 and LP5. Yellow lines represent the enhanced pathways in the knockdown and knockout lines. Pink lines represent the enhanced pathways in all BnLPAT2 transgenic lines. Blue lines represent the enhanced pathways in all BnLPAT5 transgenic lines. G3P glycerol‑3‑phosphate, LPA lysophosphatidic acid, PA phosphatidic acid, DAG diacylglycerol, TAG triacylglycerol, PC phosphatidylcholine, PE phosphatidylethanolamine, GPAT glycerol‑3‑phosphate acyltransferase, LPAT lysophosphatidate acyltransferase, DGAT diacylglycerol acyltransferase, PDAT diacylglycerol acyltransferase, DGK diacylglycerol kinase, NPC nonspecific phospholipase C, LACS long‑chain acyl‑CoA synthetase, DHAP dihydroxyacetone phosphate, OB oil body, GASA Gibberellic acid stimulated Arabidopsis, ABA abscisic acid, SDP1 sugar-dependent 1, FC fold-change, KO knockout and knockdown lines, OE overexpression lines

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