Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose

Table 1 Metabolic flux

List of metabolite	Strains	Times (induction with arabinose)
List of metabolite	Strains	0 h	2 h	4 h	6 h	8 h
D-Glucose-6P	B539	728,589	42,041,950	12,357,204	5,541,360	3,718,343
D-Glucose-6P	B5703	1,137,825	35,135,366	16,812,928	7,763,633	4,848,915
D-Fructose-1,6-2P	B539	0	111,014,765	6,182,002	894,153	612,773
D-Fructose-1,6-2P	B5703	942,182	39,481,007	3,851,501	299,258	114,375
Ribose-5P	B539	0	1,505,483	2,223,421	273,274	243,755
Ribose-5P	B5703	0	1,474,348	1,611,914	512,025	558,143
PEP	B539	126,006	22,731,977	3,331,449	2,434,459	3,248,891
PEP	B5703	380,990	1,931,877	5,752,892	5,219,953	4,746,976
Pyruvate	B539	398,717	84,632,477	58,309,713	49,395,212	77,246,204
Pyruvate	B5703	460,026	73,558,546	57,521,203	59,415,845	81,803,110
Citrate	B539	169,209	87,009,353	3,854,470	19,968,252	26,807,885
Citrate	B5703	674,282	61,961,209	8,331,791	20,782,970	31,364,602
a-Ketoglutarate	B539	93,545	904,946	4,033,714	7,213,857	20,397,767
a-Ketoglutarate	B5703	38,816	598,282	7,603,855	16,456,613	24,207,181
Fumarate	B539	4,677,244	8,186,085	4,263,780	3,333,971	3,158,021
Fumarate	B5703	2,103,875	3,600,110	7,220,976	5,007,847	4,278,011
Malate	B539	2,445,713	19,993,286	1,830,362	1,204,770	2,089,227
Malate	B5703	4,241,743	9,294,525	2,059,973	1,493,029	2,437,944
D-Mannose-6P	B539	0	14,693	29,847	0	0
D-Mannose-6P	B5703	0	0	0	0	0
GDP-D-Mannose	B539	0	0	9920	42,742	20,012
GDP-D-Mannose	B5703	0	0	0	0	0
L-Galactono-1,4-lactone	B539	1515	162,695	294,489	386,450	316,948
L-Galactono-1,4-lactone	B5703	3474	11,265	16,427	20,147	25,899
D-Galacturonate	B539	0	106,514	117,279	86,389	202,064
D-Galacturonate	B5703	0	326,280	170,989	187,455	257,359

The values are the peak areas (relative content). The values at 0 h are the relative content of the unlabeled metabolite; the values at 2 h, 4 h, 6 h, and 8 h are the relative contents of ¹³C-labeled metabolites

ISSN: 2731-3654