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Fig. 4 | Biotechnology for Biofuels and Bioproducts

Fig. 4

From: Optimum flux rerouting for efficient production of naringenin from acetate in engineered Escherichia coli

Fig. 4

Effect of fine-tuned expression of pckA under promoter variants. a Schematic diagram of pckA expression control using promoter variants with different strengths. b Naringenin titer and c specific naringenin production by engineered E. coli strains with different transcriptional levels of pckA. d Time-course culture profile of pckA-upregulated E. coli, the BNIAP109 strain. 200 mg/L p-coumaric acid and 1 mM IPTG were added when culture broths reached an OD600 of 1.0. Flask cultures were performed for 48 h in biological triplicates. Error bars indicate the standard deviations of biological triplicates. BN strain refers Escherichia coli BL21 Star™(DE3) with heterologous expression of essential enzymes for naringenin production; BNIA, BN strain with both acs overexpression and iclR knockout; BNIAP103, BNIAP113, BNIAP109, BNIAP115, BNIAP106, BNIAP104, BNIAP100 refer BNIA strain with pckA upregulation under constitutive promoter BBa_J23103, BBa_J23113, BBa_J23109, BBa_J23115, BBa_J23106, BBa_J23104, BBa_J23100, respectively; PEP phosphoenolpyruvate, TCA cycle tricarboxylic acid cycle, IPTG isopropyl β-d-thiogalactopyranoside

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