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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: Optimum flux rerouting for efficient production of naringenin from acetate in engineered Escherichia coli

Fig. 5

Culture condition optimization of the BNIAP109 strain. a Evaluation of naringenin production capacity under different conditions of IPTG concentrations and induction times. b Fermentation profile of the BNIAP109 strain under optimized culture conditions, such as induction time at OD600 of 0.8 with the addition of 0.01 mM IPTG. Flask cultures were performed for 48 h in biological triplicates. Error bars indicate the standard deviations of biological triplicates. BNIAP109 strain, Escherichia coli BL21 Star™(DE3) with heterologous expression of essential enzymes for naringenin production, acs overexpression, iclR knockout, and pckA upregulation under constitutive promoter BBa_J23109; IPTG isopropyl β-d-thiogalactopyranoside

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