Fig. 2
From: Functional mining of novel terpene synthases from metagenomes
Construction and optimization of the precursor toxicity-based functional metagenomic screening system. a Structure of pA5c–MBIS plasmid overexpressing the prenyl pyrophosphate precursors synthetic (MBIS) pathway under the control of IPTG-inducible PlacUV5. A terpene synthase mitigates the toxicity of excessive prenyl pyrophosphate precursors (red) by converting them into non-toxic terpene molecules. b Gap between growth inhibitions on the negative (green) and positive (gray) controls was maximized by adjusting the concentration of supplemented mevalonate. The MBIS operon expression was induced by 0.5 mM IPTG. Upper panel, the timepoint the strain exhibited maximum specific growth rate (μmax) with selected mevalonate concentration. Lower panel, maximum specific growth rate. c Growth profiles of negative and positive controls in the optimized screening medium containing 8 mM mevalonate