Fig. 4

Characterization of TS10F1. a TS10F1 ORF was isolated from 3 different contigs screened from a human fecal metagenome library 40301 (Additional file 1: Fig. S2). TS10F1 showed 75% identity to a histidine phosphatase family protein of Prevotella pectinovora (sky blue, correct match; blue, functional equivalence; plum, not similar amino acid). b − d Gas chromatography–mass spectrometry (GC–MS) analysis of the TS10F1 in vivo test cultures. With IPTG induction of the MBIS pathway and supplemented mevalonate, BM_TS10F1 (blue) generated more β-farnesene and its derivatives than BM_AgBis (orange) and BM_Empty (green, no IPTG induction). e Confirmation after the purification of TS10F1 (estimated size is 21.54 KDa). M, protein marker. f GC–MS analysis of in vitro enzyme assay products at a 3 h timepoint and β-farnesene standard (top)