Fig. 5
From: Functional mining of novel terpene synthases from metagenomes
Microbial production of β-farnesene from glucose via TS10F1. a Overview of biosynthesis of β-farnesene from glucose by SHE_TS10F1. Upper glycolysis was downregulated, and ZWF1 was overexpressed to shunt metabolic fluxes from glucose toward the pentose phosphate pathway to regenerate NADPH efficiently. ERG9 was also downregulated to maximize FPP availability for β-farnesene biosynthesis. ERG10 and tHMG1 were episomally overexpressed with TS10F1 to enhance the flux through the MVA pathway. b − d Comparison of culture profiles of SHE_TS10F1 and negative control SHE_Empty on glucose, including cell growth (b), glucose consumption (c), and production of major extracellular metabolites (d). e β-Farnesene and farnesoic acid production through the glucose culture of SHE_TS10F1. SHE_Empty did not produce both compounds at detectable levels