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Table 4 Thirty-eight genetic sub-networks that were partly validated by previous molecular biology studies

From: 4D genetic networks reveal the genetic basis of metabolites and seed oil-related traits in 398 soybean RILs

Subnetworks constructed in this study

Evidences from previous studies

Subnetworks constructed in this study

Evidences from previous studies

No.a

Class

Knownb

Sub-network

No.a

Class

Knownb

Sub-network

3

3D-I

New

Oil content (T)-GmDHLAT-DG(18:0/18:0)

GPDH-oil content [9]

60

4D-II

New

DG(18:0/16:0) -GmPLDγ-GmARF16-miR160b-linolenic acid (T)

GmPLDγ-oil content [16]

4

3D-I

New

Oil content (T)-GmDHLAT-DG(16:0/16:0)

GPDH-oil content [9]

61

4D-II

New

DG(16:0/16:0) -GmPLDγ-GmARF16-miR160b-linolenic acid (T)

GmPLDγ-oil content [16]

7

3D-I

Known

Linolenic acid (T)-GmCK-PC(16:0/18:3)

CK-PC [72]

62

4D-II

New

DG(18:0/18:0) -GmPLDγ-GmARF16-miR160b-linolenic acid (T)

GmPLDγ-oil content [16]

11

3D-I

New

Oleic acid (T)-GmPHS-d-glucose

GmPHS-d-glucose [69]

64

4D-II

Known

Oleic acid (T) -GmPLDα1-GmARF8a-miR167d-PI(16:0/18:2)

GmPLDα1-oleic acid [13]

19

3D-II

New

Stearic acid (T)-GmMDH1-succinic acid-GmDGAT1a

ChMDH2-oil content [19], GmDGAT1-oil content [10]

65

4D-II

Known

FA(18:0)-GmABI4-GmARF8a-miR167b-oil content (T)

ABI3-oil content [56], miR167-ARF8a [45]

20

3D-II

New

Stearic acid (T)-GmMDH1-citric acid-GmDGAT1a

ChMDH2-oil content [19], GmDGAT1-oil content [10]

78

4D-II

New

Palmitic acid (T)-GmPLDα1-GmGAMYB1-miR159a-pyruvate

GmPLDα1-oleic acid [13]

22

3D-II

New

Palmitic acids (T)-GmSPT-oleic acid-GmHAD

AtHAD-fatty acids [121]

84

4D-II

Known

TG(16:0/18:1/18:2)-GmbZIP123-GmHD-ZIPIII-10-miR166s-oil content (T)

GmbZIP123-lipid content [22], miR166-HD-ZIPIII10 [67]

24

3D-II

Known

Linolenic acid (T)-GmKASI-TG(18:1/18:2/18:3)-GmKAR

AtKAS-fatty acids [121]

86

4D-II

Known

TG(18:1/18:1/18:3)-GmTAGL-GmHD-ZIPIII-10-miR166s-oil content (T)

miR166-HD-ZIPIII10 [67]

35

3D-III

Known

Oil content (T)-GmDHLAT-GmFUM-Pyruvate

GPDH-palmitic acid [9]

90

4D-II

Known

TG(18:3/18:2/18:2)-GmFatB1-GmHD-ZIPIII-10-miR166f-oleic acid (T)

GmFatB-fatty acid [57], miR166-HD-ZIPIII10 [67]

37

3D-III

Known

Oleic acid (T)-GmSEI-GmDGAT1a-TG(16:0/18:2/18:3)

GmDGAT1-linolenic acid [9]

100

4D-II

Known

Linolenic acid (T)-GmPI4Kβ-GmHD-ZIPIII-10-miR166g-PI(16:0/18:2)

miR166-HD-ZIPIII10 [67]

38

3D-III

Known

Linoleic acid (T)-GmPDAT1-GmDGAT1a-TG(16:0/18:2/18:3)

GmDGAT1-linolenic acid [9], GmPDAT-stearic acid [12]

102

4D-II

Known

Oil content (T)-GmPLDζ2-GmHD-ZIPIII-10-miR166j-CerG1(d18:2/22:1)

GmPLDζ2-oil content [59],

miR166-HD-ZIPIII10 [67]

39

3D-III

Known

Oil content (T)-GmPLDζ2-GmDAGK-TG(18:0/16:0/18:1)

GmPLDζ2-oil content [59]

105

4D-II

Known

Stearic acid (T)-GmLEC1-b-GmHD-ZIPIII-10-miR166j-FA(18:0)

AtLEC1-stearic acid [58], miR166-HD-ZIPIII10 [67]

40

3D-III

Known

Stearic acid (T)-GmLEC1-b-GmDGAT1a-TG(18:3/18:3/18:3)

AtLEC1-stearic acid [56], GmDGAT1-linolenic acid [9]

114

4D-II

Known

FA(22:1)-GmZF351-GmHD-ZIPIII-10-miR166k-linoleic acid (T)

GmZF351-oil content [25], miR166-HD-ZIPIII10 [67]

44

3D-III

New

Palmitic acid (T)-GmBCCP1-GmFUS3-ethanolamine

AtFUS3-palmitic acid [21]

118

4D-II

New

Stearic acid (T)-GmABI3b-GmNLP6-miR1516b-palmitic acid

ABI3-oil content [56]

45

3D-III

Known

Linolenic acid (T)-GmKAR-GmPK-leucine

AtPK-linoleic acid [6]

119

4D-II

New

Stearic acid (T)-GmLEC1-b-GmNLP6-miR1516b-palmitic acid

AtLEC1-stearic acid [56]

49

4D-I

New

Linoleic acid (T)-GmABI4-miR530d-TG(18:1/18:1/18:1)

ABI3-oil content [56]

121

4D-II

New

PE(16:0/18:1)-GmDof11-GmNLP6-miR1516b-palmitic acid (T)

GmDof11-lipid content [26]

53

4D-I

New

Linolenic acid (T)-GmOBO-miR5036-TG(16:0/16:0/18:1)

OBO-miR5036 [66]

125

4D-II

New

LPC(16:0)-GmDREBL-GmTCP-miR319h-linolenic acid (T)

GmDREBL-lipid content [24]

55

4D-II

New

Oleic acid (T)-GmPLDα1-GmARF16-miR160d-TG(18:0/16:0/18:1)

GmPLDα1-oleic acid [13]

127

4D-II

New

Linolenic acid (T)-GmbZIP123-GmTCP-miR319h-TG(16:0/18:1/18:2)

GmbZIP123-lipid content [22]

59

4D-II

New

Stearic acid (T)-GmLEC1-b-GmARF16-miR160d-TG(18:0/18:1/18:1)

AtLEC1-stearic acid [56]

128

4D-II

New

Oil content (T)-GmDHLAT-GmTCP2-miR319f-TG(18:1/18:1/18:3)

GPDH-oil content [9]

  1. aThe number of sub-networks in Additional file 1: Table S21
  2. b‘Known’ subnetworks could be found at the KEGG PATHWAY website (https://www.kegg.jp/kegg/pathway.html) and ‘new’ subnetworks were constructed in this study