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Table 1 Strains and plasmids used in this study

From: Both levoglucosan kinase activity and transport capacity limit the utilization of levoglucosan in Saccharomyces cerevisiae

Strains and plasmids

Genotype

Source

CEN.PK 113-5D

MATa; ura3-53

[25]

CEN-pJFE3

CEN.PK 113-5D derivative; pJFE3

This work

CEN-Rho

CEN.PK 113-5D derivative; pJFE3-Rho

This work

CEN-Sch

CEN.PK 113-5D derivative; pJFE3- Sch

This work

CEN-Mey

CEN.PK 113-5D derivative; pJFE3- Mey

This work

CEN-Lip

CEN.PK 113-5D derivative; pJFE3- Lip

This work

CEN-Asp

CEN.PK 113-5D derivative; pJFE3- Asp

This work

CEN-Koc

CEN.PK 113-5D derivative; pJFE3- Koc

This work

EBY.VW4000

MATα leu2-3,112 ura3-52 trp1-289 his3-Δ1 Mal2-8c SUC2 hxt17Δ hxt13Δ::loxP hxt15Δ::loxP hxt16Δ::loxP hxt14Δ::loxP hxt12Δ::loxP hxt9Δ::loxP hxt11Δ::loxP hxt10Δ::loxP hxt8Δ::loxP hxt514::loxP hxt2Δ::loxP hxt367Δ::loxP gal2Δ stl1Δ::loxP agt1Δ::loxP ydl247wΔ::loxP yjr160cΔ::loxP

[28]

EBY-pIYC04

EBY.VW4000 derivative; pIYC04

This work

EBY-pIYC04-pJFE3

EBY-pIYC04 derivative; pJFE3

This work

YLGR000

EBY.VW4000 derivative; pIYC04-Rho

This work

YLGR00P

YLGR00 derivative; pJFE3

This work

YLGR00G

YLGR00 derivative; pJFE3-GAL2WT

This work

YLGR085

YLGR00 derivative; pJFE3-GAL2F85A

This work

YLGR215

YLGR00 derivative; pJFE3-GAL2Q215A

This work

YLGR218

YLGR00 derivative; pJFE3-GAL2I218A

This work

YLGR341

YLGR00 derivative; pJFE3-GAL2Q341A

This work

YLGR342

YLGR00 derivative; pJFE3-GAL2Q342A

This work

YLGR346

YLGR00 derivative; pJFE3-GAL2N346A

This work

YLGR347

YLGR00 derivative; pJFE3-GAL2N347A

This work

YLGR350

YLGR00 derivative; pJFE3-GAL2F350A

This work

YLGR446

YLGR00 derivative; pJFE3-GAL2Y446A

This work

YLGR455

YLGR00 derivative; pJFE3-GAL2W455A

This work

YLGR2M

YLGR00 derivative; pJFE3-GAL2 Q341A W455A

This work

pJFE3

2 μ expression vector with URA3 marker, TEF1 promoter, PGK1 terminator

[24]

pJFE3-Rho

Coding gene of 1,6-anhydro-N-acetylmuramic acid kinase from Rhodotorula toruloides cloned into pJFE3

This work

pJFE3- Sch

Coding gene of 1,6-anhydro-N-acetylmuramic acid kinase from Scheffersomyces stipites cloned into pJFE3

This work

pJFE3- Mey

Coding gene of 1,6-anhydro-N-acetylmuramic acid kinase from Meyerozyma guilliermondii cloned into pJFE3

This work

pJFE3- Lip

Coding gene of levoglucosan kinase from Lipomyces starkeyi cloned into pJFE3

This work

pJFE3- Koc

Coding gene of levoglucosan kinase from Kockovaella imperatae cloned into pJFE3

This work

pJFE3- Asp

Coding gene of 1,6-anhydro-N-acetylmuramic acid kinase from Aspergillus niger cloned into pJFE3

This work

pIYC04

Yeast 2μ plasmid, PGK1p-CYC1t, TEF1p-ADHt, HIS3 marke

[27]

pIYC04-Rho

Coding gene of 1,6-anhydro-N-acetylmuramic acid kinase from Rhodotorula toruloides cloned into pIYC04

This work

pIYC04-Sch

Coding gene of 1,6-anhydro-N-acetylmuramic acid kinase from Scheffersomyces stipites cloned into pIYC04

This work

pJFE3-GAL2WT

pJFE3-TEF1p-GAL2-PGK1t

This work

pJFE3-GAL2F85A

pJFE3-TEF1p-GAL2F85A-PGK1t

This work

pJFE3-GAL2Q215A

pJFE3-TEF1p-GAL2Q215A-PGK1t

This work

pJFE3-GAL2I218A

pJFE3-TEF1p-GAL2I218A-PGK1t

This work

pJFE3-GAL2Q341A

pJFE3-TEF1p-GAL2Q341A-PGK1t

This work

pJFE3-GAL2Q342A

pJFE3-TEF1p-GAL2Q342A-PGK1t

This work

pJFE3-GAL2N346A

pJFE3-TEF1p-GAL2N346A-PGK1t

This work

pJFE3-GAL2N347A

pJFE3-TEF1p-GAL2N347A-PGK1t

This work

pJFE3-GAL2F350A

pJFE3-TEF1p-GAL2F350A-PGK1t

This work

pJFE3-GAL2Y446A

pJFE3-TEF1p-GAL2Y446A-PGK1t

This work

pJFE3-GAL2W455A

pJFE3-TEF1p-GAL2W455A-PGK1t

This work

pJFE3-GAL2Q341AW455A

pJFE3-TEF1p-GAL2 Q341A W455A-PGK1t

This work