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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Construction and yield optimization of a cinnamylamine biosynthesis route in Escherichia coli

Fig. 3

Construction of cinnamylamine biosynthetic pathway using cinnamic acid as the precursor in E. coli. A The two biosynthetic pathways from cinnamic acid to cinnamylamine and plasmid maps. 4CL: 4-coumarate: CoA ligase. CCR: cinnamyl-CoA reductase. CAR: carboxylic acid reductase. PPTase: phosphopantetheinyl transferase. PtrCCR: cinnamoyl-CoA reductase from P. trichocarpa. Ptr4CL: 4-coumarate: CoA ligase from P. trichocarpa. Sc4CL: 4-coumarate: CoA ligase from S. coelicolor. AtCCR: cinnamoyl-CoA reductase from A. thaliana. Cv-ωTA: ω-transaminase from C. violaceum. NcCAR: carboxylic acid reductase from N. crassa. NiCAR: carboxylic acid reductase from N. iowensis. PPTase: phosphopantetheinyl transferase from E. coli. B Accumulation of various substances in the cinnamylamine production pathway of the MG1655 (RARE) strain using cinnamic acid as the precursor with four different cinnamylamine production plasmids (petP, petE, petS, and petI). Data represent the mean ± SD (error bars) from three independent experiments

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