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Fig. 1 | Biotechnology for Biofuels and Bioproducts

Fig. 1

From: An engineered non-oxidative glycolytic bypass based on Calvin-cycle enzymes enables anaerobic co-fermentation of glucose and sorbitol by Saccharomyces cerevisiae

Fig. 1

Schematic representation of NADH redox-cofactor balances during glucose fermentation by wild-type S. cerevisiae (left) and during sorbitol fermentation by an engineered PRK-RuBisCO expressing strain (right). Coloured boxes indicate pathways used for anaerobic reoxidation of NADH generated in biosynthesis. The red box indicates the native S. cerevisiae glycerol pathway for NADH reoxidation, while the green box indicates the engineered PRK-RuBisCO bypass. in the last scenario, re-oxidation of ‘surplus’ NADH generated during biomass formation and/or sorbitol fermentation is coupled to ethanol production. This scenario involves an engineered strain expressing a membrane transporter that enables energy-independent uptake of sorbitol, together with an NAD+-dependent sorbitol dehydrogenase and an optimized PRK-RuBisCO pathway. GPD2: NAD+-dependent glycerol-3-phosphate dehydrogenase; PRK: ribulose-5-phosphate kinase; RuBisCO: ribulose-1,5-bisphosphate carboxylase/oxygenase

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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