Fig. 3

Growth, glucose consumption, sorbitol consumption, ethanol formation and glycerol formation in anaerobic bioreactor batch cultures of S. cerevisiae strains IME324 (reference strain) (A), IME611 (overexpression cassettes for HXT15 and SOR2) (B), IMX1489 (optimized PRK-RuBisCO bypass and gpd2∆ mutation, [26]) (C) and IMX2506 (optimized PRK-RuBisCO bypass and gpd2∆ mutation, overexpression cassettes for HXT15 and SOR2) (D). Cultures were grown anaerobically at pH 5 and at 30 °C on synthetic medium containing 20 g L⁻¹ glucose and 30 g L⁻¹ sorbitol as carbon sources