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Fig. 5 | Biotechnology for Biofuels and Bioproducts

Fig. 5

From: An engineered non-oxidative glycolytic bypass based on Calvin-cycle enzymes enables anaerobic co-fermentation of glucose and sorbitol by Saccharomyces cerevisiae

Fig. 5

Sorbitol consumption (A) and glycerol production (B) in duplicate anaerobic chemostat cultures of strain IMX2506 (optimized PRK-RuBisCO bypass and gpd2∆ mutation, overexpression cassettes for HXT15 and SOR2). For the first 20 generations, the cultures were grown on a mixture of 10 g L−1 sorbitol and 10 g L−1 glucose at a dilution rate of 0.05 h−1. After 20 h, the dilution rate was decreased to 0.025 h−1 and after 50 generations the substrate mixture was changed to 20 g L−1 sorbitol and 10 g L−1 glucose

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