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Table 3 S. cerevisiae strains used in this study

From: An engineered non-oxidative glycolytic bypass based on Calvin-cycle enzymes enables anaerobic co-fermentation of glucose and sorbitol by Saccharomyces cerevisiae

Strain name

Relevant genotype

Parental strain

Reference

CEN.PK113-7D

MATa URA3 GPD2

-

[49]

IMX581

MATa ura3-52 GPD2 can1::cas9-natNT2

CEN.PK113-5D

[51]

IME324

MATa ura3-52 GPD2 can1::cas9-natNT2 p426-TEF (URA3)

IMX581

[26]

IMX1489

MAT2 ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1-tRPE1, pPGK1-TKL1-tTKL1, pTEF1-TAL1-tTAL1, pPGI1-NQM1-tNQM1, pTPI1-RKI1-tRKI1, pPYK1-TKL2-tTKL2 sga1::pDAN1-prk, pTDH3-cbbm-tCYC1 (9 copies), pTPI1-groES-tPGI1, pTEF1-groEL-tACT1 pUDR103 (KlURA3)

IMX581

[26]

IMX2411

MATa ura3-52 GPD2 can1::cas9-natNT2 X-2::pTEF1-HXT13-tCYC1, pACT1-SOR2-tCPS1

IMX581

This study

IMX2495

MAT2 ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1-tRPE1, pPGK1-TKL1-tTKL1, pTEF1-TAL1-tTAL1, pPGI1-NQM1-tNQM1, pTPI1-RKI1-tRKI1, pPYK1-TKL2-tTKL2 sga1::pDAN1-prk, pTDH3-cbbm-tCYC1 (9 copies), pTPI1-groES-tPGI1, pTEF1-groEL-tACT1 X-2::pTEF1-HXT13-tCYC1, pACT1-SOR2-tCPS1

IMX1489

This study

IMX2506

MAT2 ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1-tRPE1, pPGK1-TKL1-tTKL1, pTEF1-TAL1-tTAL1, pPGI1-NQM1-tNQM1, pTPI1-RKI1-tRKI1, pPYK1-TKL2-tTKL2 sga1::pDAN1-prk, pTDH3-cbbm-tCYC1 (9 copies), pTPI1-groES-tPGI1, pTEF1-groEL-tACT1 X-2::pTEF1-HXT13-tCYC1, pACT1-SOR2-tCPS1 p426-TEF (URA3)

IMX2495

This study

IME611

MATa ura3-52 GPD2 can1::cas9-natNT2 X-2::pTEF1-HXT13-tCYC1, pACT1-SOR2-tCPS1 p426-TEF (URA3)

IMX2411

This study